Looking for the definition of ECORI?
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Looking for online definition of ECOR or what ECOR stands for?
ECOR is listed in the World’s largest and most authoritative dictionary database of abbreviations and acronyms The Free Dictionary, EcoRV (pronounced “eco R five”) is a type II restriction endonuclease isolated from certain strains of Escherichia coli.
It has the alternative name Eco32I.
In molecular biology, it is a commonly used restriction enzyme.
It creates blunt ends.
The enzyme recognizes the palindromic 6-base DNA sequence 5′-GAT|ATC-3′ and makes a cut at the vertical line.
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Meaning of ECORI.
What does ECORI mean?
Information and translations of ECORI in the most comprehensive dictionary definitions resource on #, R.
Steeves, in Calcium-Binding Proteins in Health and Disease, 1987 Assay of calbindin mRNA The calbindin cDNA has been used to develop a quantitative assay for the mRNA for this protein by inserting it into the EcoRI -Sall site of the multiple cloning sequence of #, What does ‘H’ in BamHI stand for?
Ask Question Asked 5 years, 11 months ago.
Active 5 years, 11 months ago.
Viewed 3k times 3 $begingroup$ It’s not explained on the wikipedia page.
So if, apparently, ‘H’ is not relevant, why is it part of the name?
And if it is relevant, why is it not explained?
Clone the HEXA cDNA into the XhoI/ EcoRI sites of pEGFP-N1 expression plasmid (BD Clontech) in the correct orientation and in frame with EGFP sequence.
One day before the transfection, seed 3 10 5 cells/well (6-well plate) in 2 ml of growth medium.
Transfect HEK293 cells with 1 g/well of pEGFP-N1-HEXA or the empty pEGFP-N1 vector (Mock) using lipofectamine LTX reagent.
HindIII, Restriction Enzyme, BamHI, Polymerase chain reaction, EcoRV